RESUMO
Abstract Purpose: To establish a new rat model, the pathogenesis of which is closer to the clinical occurrence of chronic obstructive jaundice with liver fibrosis. Methods: 90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology. Results: Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group. Conclusion: Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis.
Assuntos
Animais , Feminino , Ductos Biliares/efeitos dos fármacos , Modelos Animais de Doenças , Géis/administração & dosagem , Cirrose Hepática/induzido quimicamente , Aspartato Aminotransferases/sangue , Valores de Referência , Compostos Azo , Fatores de Tempo , Ductos Biliares/patologia , Bilirrubina/análise , Albumina Sérica/análise , Ensaio de Imunoadsorção Enzimática , Imuno-Histoquímica , Distribuição Aleatória , Reprodutibilidade dos Testes , Ratos Sprague-Dawley , Amarelo de Eosina-(YS) , Icterícia Obstrutiva/induzido quimicamente , Icterícia Obstrutiva/patologia , Fosfatase Alcalina/sangue , gama-Glutamiltransferase/sangue , Injeções , Cirrose Hepática/patologia , Verde de MetilaRESUMO
Hyperthyroidism is one of the most frequent endocrine disorders and its current treatment is based on drugs, surgery and radioactive iodine. Methimazole is the antithyroid drug of choice because of its potency and infrequent side effects, usuaIly mild. This medication is rarely associated with liver toxicity, usually manifested as cholestatic jaundice. Here we report the case of a 33-year-old woman treated at the University Hospital Fundación Santa Fe de Bogota, with hepatotoxicity induced by a methimazole-based treatment for Graves disease. The pruritus and jaundice appeared after three weeks of therapy, viral hepatitis markers were negative, hepatobiliary ultrasonography was normal, and an increase of the levels of alkaline phosphatase, total bilirubin and aminotransferases was found The causal diagnosis of methimazole-induced hepatotoxicity was supported by the results of a liver biopsy. According to the CIOMS scale the score was 10, and the causal relationship of the hepatic adverse reaction by methimazole is highly probable. The clinical course was satisfactory when the medication was suspended, with clinical improvement at 5 days, and normalization of liver tests at 5 weeks. We discuss this case from a diagnostic and therapeutic approach.
Assuntos
Antitireóideos/efeitos adversos , Icterícia Obstrutiva/induzido quimicamente , Metimazol/efeitos adversos , Adulto , Feminino , Hipertireoidismo/tratamento farmacológico , Humanos , Icterícia Obstrutiva/diagnósticoRESUMO
Liver injury occurs with many drugs; therefore, a thorough work up is important for establishing the diagnosis. We report a case of trifluoperazine-induced cholestatic jaundice. A 44-year old male with schizoaffective disorder developed an increase in liver enzymes and jaundice after starting treatment with trifluoperazine .Workup for other potential etiologies was negative